This kit supplies CellMatrix™ Basement Membrane Gel and cell culture components to support a serum-free, feeder-free cell culture environment, including Pluripotent Stem Cell SFM XF/FF (ATCC ACS-3002), CellMatrix Basement Membrane Gel (ATCC ACS-3035), Stem Cell Dissociation Reagent (ATCC ACS-3010), ROCK Inhibitor Y27632 (ATCC ACS-3030), Stem Cell Freezing Media (ATCC ACS-3020), DMEM: F12 Medium (ATCC 30-2006)and D-PBS (ATCC 30-2200).